Use of autologous adipose-derived mesenchymal stem cells for creation of laryngeal cartilage.

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Use of autologous adipose-derived mesenchymal stem cells for creation of laryngeal cartilage.

Laryngoscope. 2017 Dec 14;:

Authors: Zhang H, Voytik-Harbin S, Brookes S, Zhang L, Wallace J, Parker N, Halum S

Abstract
OBJECTIVES/HYPOTHESIS: Adipose-derived mesenchymal stem cells (ASCs) are an exciting potential cell source for tissue engineering because cells can be derived from the simple excision of autologous fat. This study introduces a novel approach for tissue-engineering cartilage from ASCs and a customized collagen oligomer solution, and demonstrates that the resultant cartilage can be used for laryngeal cartilage reconstruction in an animal model.
STUDY DESIGN: Basic science experimental design.
METHODS: ASCs were isolated from F344 rats, seeded in a customized collagen matrix, and cultured in chondrogenic differentiation medium for 1, 2, and 4 weeks until demonstrating cartilage-like characteristics in vitro. Large laryngeal cartilage defects were created in the F344 rat model, with the engineered cartilage used to replace the cartilage defects, and the rats followed for 1 to 3 months. Staining examined cellular morphology and cartilage-specific features.
RESULTS: In vitro histological staining revealed rounded chondrocyte-appearing cells evenly residing throughout the customized collagen scaffold, with positive staining for cartilage-specific markers. The cartilage was used to successfully repair large cartilaginous defects in the rat model, with excellent functional results.
CONCLUSIONS: This study is the first study to demonstrate, in an animal model, that ASCs cultured in a unique form of collagen oligomer can create functional cartilage-like grafts that can be successfully used for partial laryngeal cartilage replacement.
LEVEL OF EVIDENCE: NA Laryngoscope, 2017.

PMID: 29238978 [PubMed - as supplied by publisher]

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