CREPT overexpression is associated with proliferation behaviors and poor prognosis in non-small cell lung cancer

Summary

The cell-cycle-related and expression-elevated protein in tumor (CREPT) is overexpressed in several human malignancies. However, the clinical relevance of CREPT expression and its biological role in non-small cell lung cancer (NSCLC) remains unclear.In this study, we detected the expression of CREPT in both NSCLC tissues and cell lines by immunohistochemistry, Western Blot and RT-PCR. The correlation between CREPT expression and clinical pathologic features was analyzed in 271 NSCLC patients. Prognostic value of CREPT expression was evaluated by Kaplan-Meier analysis and Cox regression analysis. CREPT was overexpressed in Calu-1 cell lines by using plasmid vector and its biological function was explored both in vitro and in vivo. We found that CREPT was significantly overexpressed in NSCLC compared with paired adjacent non-tumor tissues, and the expression level of CREPT was correlated with tumor differentiation, lymph node metastasis and clinical stage. Kaplan-Meier analysis showed that the RFS and OS of high CREPT expression groups were significantly shorter than those of the low CREPT expression group. Multivariate analysis was identified that CREPT might be an independent biomarker for the prediction of NSCLC prognosis. Overexpression of CRPET increased cell proliferation enhanced the migration and invasion ability of Calu-1 cells (a human NSCLC cell line with relative low CRPET expression) in vitro. Moreover, CREPT overexpression promoted tumor growth in nude mice model. These results suggest that CREPT is closely relevant to the proliferation of NSCLC cells and it might be a potential prognostic marker in NSCLC patients.

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from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2GGej7n