Abstract
Aim
A number of homeobox transcriptional factors are utilized as organ-specific markers in the histopathological diagnosis of neoplasms. We have screened a homeobox gene that is specifically expressed in normal sweat gland cells and is useful for the histopathological diagnosis of sweat gland neoplasms.
Methods and results
By screening an open database resource of The Human Protein Atlas, 37 genes among the 235 homeobox transcriptional factors were found to be specifically expressed in the skin. Among those 37 genes, the engrailed homeobox 1 (En1) was expressed in normal eccrine glands but not in the epidermal keratinocytes. Expression of En1 was found throughout the eccrine glands, but not in the apocrine secretory coils, sebaceous glands, or hair follicles. Expression of En1 was immunohistochemically examined in 111 cases of cutaneous epithelial neoplasms. All the 9 cases of poroma, 7 cases of spiradenoma, and 6 cases of syringoma, which are considered to differentiate toward eccrine glands, showed positive nuclear staining in most of the tumour cells. Sebaceous gland and hair follicle tumours were immuno-negative. En1 was focally expressed in the epidermal neoplasms of seborrheic keratosis and squamous cell carcinoma.
Conclusion
En1 was specifically expressed in normal eccrine glands and was expressed in most of the tumour cells of sweat gland neoplasms with eccrine gland differentiation. En1 was focally expressed in epidermal neoplasms, however, it was absent in sebaceous or hair follicle neoplasms. These findings will help in the histopathological diagnosis as well as to understand the histogenesis of sweat gland neoplasms.
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from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2EyZsO3
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