Can Hyaluronidase Be an Alternative Postoperative Anti-edema Agent to Dexamethasone? Preliminary Results of an Animal Study

Publication date: Available online 21 March 2018
Source:Journal of Oral and Maxillofacial Surgery
Author(s): K.O.Ç. Onur, E.R. Nuray
PurposeRecombinant human hyaluronidase (rHuPH20) is widely used as a spreading factor, which enhances the absorption of subcutaneously injected medicines. The anti-inflammatory and anti-edema effects of the enzyme were demonstrated in studies before. In the present study, the anti-edema effect of rHuPH20 was compared with that of dexamethasone in a traumatic rat paw edema model.MethodsTwenty-four Sprague–Dawley rats (200-450 g) were divided into three groups, as follows: control (group I), rHuPH20 (group II), and dexamethasone (group III). Traumatic edema was induced in the right hind paws of the rats using Feeney's weight-drop model. After edema induction, 0.4 mL rHuPH20 (100 U/kg = 0.88 μg/kg dose) and 0.4 mL dexamethasone (0.5 mg/kg dose) were injected into the right hind paws of the rats in groups II and III. Paw volumes were measured before edema induction and 3, 6, 12, 24, 48, and 72 hours after induction using a plethysmometer. The Mann-Whitney U test was used for statistical analyses. Probabilities < 0.05 were accepted as significant.ResultsBetween percentage change in edema mean values of groups I and III, there was no significant difference at all time points, but group II has significantly less change in edema mean values 3, 6, 12, 24, and 48 hours after edema induction (p<0.05) when compared with group I. The change in edema mean values of group II were significantly less than that of group III at 3, 6, 12, 24, and 48 hours after edema induction (p<0.05).ConclusionLocal rHuPH20 injection more effectively reduces edema that was constituted traumatically in rat paws than does dexamethasone. But, further clinical studies must be performed for the use of hyaluronidase enzyme as a postoperative anti-edema agent instead of dexamethasone.



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