Detection of GSK-3β activation index in pediatric chronic tonsillitis is an indicator for chronic recurrent inflammation

Publication date: Available online 3 March 2018
Source:American Journal of Otolaryngology
Author(s): Gao Yunfei, Mi Jiaoping, Chen Fenghong, Liao Zhenpeng, Feng Xiaoshan, Lv Minghui, He Haixin, Cao Yujie, Yan Yan, Zhu Zhe, Fan Yunping, Hong Haiyu
PurposeChronic tonsillitis (TC) is among the most common bacterial diseases in pediatric otolaryngology. We aimed to evaluate the expression of glycogen synthase kinase 3β (GSK-3β) in a cohort of children with chronic tonsillitis (TC), and the correlation between GSK-3β activity index and inflammatory profiles of TC.Materials and methodsThe expression of GSK-3β was comparably evaluated between children with TC (n = 26) and tonsillar hypertrophy (TH, n = 26). GSK-3β expression was detected by immunohistochemistry, RT-qPCR, and Western blot. The inflammatory profiles between the TC and TH groups were also evaluated.ResultsWe found that while GSK-3β was highly expressed in both TC and TH groups, no significant difference were detected at mRNA and protein levels between groups. The protein level of p-GSK-3β was significantly lower in the TC group as compared to the TH group. Additionally, the inflammatory markers, including NF-κB, T-bet, and IFN-γ were higher in the TC group compared to TH group. The GSK-3β activation index was positively correlated with the levels of NF-κB, T-bet, and IFN-γ in the TC group.ConclusionsOur findings suggested that GSK-3β activation index was demonstrated to be a clinically applicable indicator for chronic recurrent inflammation in pediatric TC.



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