Genotypic characterization of Streptococcus mutans in Child-mother pair − A PCR based study

Publication date: Available online 8 September 2017
Source:Journal of Oral Biology and Craniofacial Research
Author(s): Dhanalakshmi Ravikumar, Mahesh Ramakrishna, Sharna Ningthoujam, Waikhom Robindro, Gayathri R, Vishnu Priya V
ObjectivesAt present, there is lack of evidence in literature regarding the transmission of Streptococcus Mutans from a caries-free mother to child by genetic methods, so the present study aimed to identify the genetic characterization of Streptococcus Mutans strains isolated from caries-free and caries active children and their mother being caries-free in both the groups and also to identify the genetic patterns of Streptococcus Mutans between caries-free and caries-active individuals.MethodsTwenty child-mother pair were selected and divided into 2 groups and the mothers being caries-free in both the groups. Saliva samples were collected using a sterile tube, followed by microbial culture of Streptococcus mutans, DNA isolation and PCR amplification. The molecular weights of each band were converted into a binary data and data were entered into SPSS software version 20.0 to generate similarity dendrograms.ResultsAmplified products of Streptococcus Mutans demonstrated a same genetic distance between the mother-child pair, indicative of a closely related species. Dendrogram interpretation represented a greater genetic polymorphism of Streptococcus Mutans between caries-free and caries-active children.ConclusionsIdentical genetic distance between child-mother pair showed that, these Streptooccus Mutans were closely related and could have vertically transmitted from their mothers. Different genotypic pattern between caries-free and caries active subjects showed a genetic polymorphism among the Streptococcus Mutans strains.



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