Αρχειοθήκη ιστολογίου

Τρίτη 3 Οκτωβρίου 2017

Rostro-caudal maturation of glial cells in the accessory olfactory system during development: involvement in outgrowth of GnRH neurites

Abstract

During mammalian embryonic development, GnRH neurones differentiate from the nasal placode and migrate through the nasal septum toward the forebrain. We previously showed that a category of glial cells, the olfactory ensheathing cells (OEC), forms the microenvironment of migrating GnRH neurones. Here, to caraterize the quantitative and qualitative importance of this glial, we investigated the spatio-temporal maturation of glial cells in situ and the role of maturing glia in GnRH neurones development ex vivo. More than 90% of migrating GnRH neurones were found to be associated with glial cells. There was no change in the cellular microenvironment of GnRH neurones in the regions crossed during embryonic development as glial cells formed the main microenvironment of these neurones (53.4%). However, the phenotype of OEC associated with GnRH neurones changed across regions. The OEC progenitors immunoreactive to brain lipid binding protein formed the microenvironment of migrating GnRH neurones from the vomeronasal organ to the telencephalon and were also present in the diencephalon. However, during GnRH neurone migration, maturation of OEC to [GFAP+] state (glial fibrillary acid protein) was only observed in the nasal septum. Inducing depletion of OEC in maturation, using transgenic mice expressing herpes simplex virus thymidine kinase driven by the Gfap promoter, had no impact on neurogenesis or on triggering GnRH neurone migration in nasal explant culture. Nevertheless, depletion of [GFAP+] cells decreased GnRH neurite outgrowth by 57.4%. This study suggests that specific maturation of OEC in the nasal septum plays a role in morphological differentiation of GnRH neurones.

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from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2xS4sre

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