Tumor-derived exosomes enhance invasion and metastasis of salivary adenoid cystic carcinoma cells

Abstract

Objectives

Tumor-derived exosomes (TDE) have been shown to participate in different steps of the dissemination of cancer cells. However, the role of salivary adenoid cystic carcinoma-derived (SACC-derived) exosomes had not been documented in SACC. The study aims to explore the functions of SACC-derived TDE in SACC progression and investigate potential mechanisms.

Methods

SACC cell line SACC-83 was used to generate TDE. Afterwards, SACC-83 or HUVECs was co-cultured with or without TDE. Tumor migration, tumor invasion, and endothelial permeability were examined by wound healing assay, tumor invasion assay, endothelial permeability assay, and tumor cell transendothelial migration assay, respectively. Moreover, the expression levels of cell junction related proteins were examined by qRT-PCR and Western Blot.

Results

SACC-83-derived exosomes were taken up by their host cells. Meanwhile, TDE increased migration and invasion capacity of SACC-83 cells and enhanced endothelial cell permeability. Furthermore, we demonstrated that the expression of cell junction related proteins (Claudins and ZO-1) was down-regulated, which is presumably involved in the TDE-mediated promotion of migration, invasion and metastasis.

Conclusion

The results suggested that SACC cells-derived exosomes were loaded with individual components that could enhance invasiveness and induce microenvironment changes, thus promoting SACC aggression.

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from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2z0xE2O