Αρχειοθήκη ιστολογίου

Παρασκευή 24 Νοεμβρίου 2017

Oral sampling methods are associated with differences in immune marker concentrations

Objective

To determine whether the concentration and distribution of immune markers in paired oral samples were similar.

Study Type

Clinical research.

Study Design

Cross-sectional study.

Methods

Paired saliva and oral secretions (OS) samples were collected. The concentration of immune markers was estimated using Luminex multiplex assay (Thermo Fisher Scientific, Waltham, MA). For each sample, the concentration of respective immune markers was normalized to total protein present and log-transformed. Median concentrations of immune markers were compared between both types of samples. Intermarker correlation in each sampling method and across sampling methods was evaluated.

Results

There were 90 study participants. Concentrations of immune markers in saliva samples were significantly different from concentrations in OS samples. Oral secretions samples showed higher concentrations of immunoregulatory markers, whereas the saliva samples contained proinflammatory markers in higher concentration.

Conclusion

The immune marker profile in saliva samples is distinct from the immune marker profile in paired OS samples.

Level of Evidence

2b. Laryngoscope, 2017



from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2A7Gmd0

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