Characteristics and Responses of Human Vocal Fold Cells in a Vibrational Culture Model

Objectives/Hypothesis

This study was conducted to provide a vibrational culture model to investigate the effects of mechanical environments on cellular functions, and elucidate physiological characteristics of two different types of cells in vocal folds under static and vibrational conditions.

Study Design

In vitro study of human vocal fold fibroblasts (hVFFs) and human macula flava stellate cells (hMF-SCs).

Methods

hVFFs and hMF-SCs were exposed to a 2-second-on/2-second-off, 205 Hz vibration regime for 4 hours by using a vibrational culture model. We compared cell morphology, cell viability, and gene expression in extracellular matrix–related components, growth factors, and differentiation markers under static and vibratory conditions.

Results

hVFFs and hMF-SCs differed in their morphologies and gene expression levels under static condition. The applied vibration did not induce changes in morphology and viability of either cell type. However, gene expression levels changed in both cell types in response to vibration; in particular, hMF-SCs exhibited a more sensitive response to vibration than that shown by hVFFs.

Conclusions

The vibrational culture model developed in this study enabled us to investigate the effects of the applied vibration on two types of vocal fold resident cells. As a result, we could demonstrate that hVFFs and hMF-SCs exhibited distinctively different characteristics under vibrational conditions.

Level of Evidence

NA Laryngoscope, 2018

from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2nsPB2v