Evaluating the Effect of Resveratrol on the Healing of Extraction Sockets in Cyclosporine A-treated rats

Publication date: Available online 8 March 2018
Source:Journal of Oral and Maxillofacial Surgery
Author(s): Ayse Ozcan Kucuk, Hilal Alan, Mehmet Gul, Umit Yolcu
PurposeThe objective of this study was to investigate the effects of resveratrol on alveolar socket healing after tooth extraction in normal and cyclosporin A (CsA)-treated rats.Materials and MethodsSeventy-two female Sprague Dawley rats were separated into four groups of 18. Group 1 was injected with a placebo solution intraperitoneally. Group 2 was injected with resveratrol (10 μmol/kg) intraperitoneally. Groups 3 and 4 were injected with CsA (10 mg/kg) subcutaneously for 8 days once daily before the tooth extraction. Next, the teeth were extracted and continued to CsA injection until the animals were sacrificed. Eight days after commencing the CsA injections, Group 4 was injected with resveratrol while continuing with CsA injections. Nine rats from each group were sacrificed on days 14 and 28, and sections were examined to assess the degree of inflammation, the formation of connective tissue, and new bone formation. Immunohistochemical analysis was employed to evaluate the alveolar socket healing process using osteocalcin and osteopontin markers; p<0.05 was considered significant.ResultsThere was more new bone formation in Group 2 than in the other three groups on day 14 after the tooth extraction (p<0.05), and there was more new bone formation in Group 2 than in Groups 3 and 4 on day 28 after the extraction (p<0.05). Based on the immunohistochemical assessment, the amount of osteocalcin and osteopontin labelling was higher in Group 2 compared to the other three groups on day 14 (p<0.05); however, on day 28 after the extraction it was higher in Group 4 compared to Group 3 (p<0.05).ConclusionsResveratrol improves alveolar socket healing in normal and CsA-treated rats. Resveratrol also increases the levels of osteocalcin and osteopontin in normal and CsA-treated rats. These results suggest that the use of this natural compound is useful for alveolar socket healing after tooth extraction.



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