Circulating microRNAs in Graves' Disease in Relation to Clinical Activity.

Circulating microRNAs in Graves' Disease in Relation to Clinical Activity.

Thyroid. 2016 Sep 9;

Authors: Hiratsuka I, Yamada H, Munetsuna E, Hashimoto S, Itoh M

Abstract
BACKGROUND: Understanding the roles of circulating microRNAs (miRNAs) can provide important and novel information regarding disease pathogenesis and the patient's clinical condition. Circulating miRNAs, such as exosomal miRNA, may regulate various bioactivities related to intercellular communications. However, the circulation of miRNAs in Graves' disease (GD) in relation to disease activity has never been elucidated. This study aimed to identify circulating miRNAs in GD in relation to disease activity and whether their exosomes play a role in the pathogenesis of GD.
METHODS: Circulating miRNAs were measured in serum obtained from 7 intractable GD patients, 7 GD patients in remission, and 7 healthy controls using the miScript miRNA PCR Array. Altered miRNAs selected from array data were validated in 65 subjects. To investigate exosome biology, peripheral blood mononuclear cells (PBMCs) were incubated with exosomes isolated from sera of the subjects. We quantified mRNAs for cytokines using quantitative real-time PCR.
RESULTS: Circulating miR-23b-5p and miR-92a-39 were increased in GD patients in remission compared with intractable GD patients (P < 0.05). On the other hand, let-7g-3p and miR-339-5p were decreased in GD patients in remission compared with intractable GD patients (P < 0.05). Exosomes from intractable GD patients stimulated mRNA expression for IL-1 and TNF-α compared with GD patients in remission or healthy controls.
CONCLUSIONS: We demonstrate that different levels of circulating miRNAs are associated with the intractable GD. Moreover, serum exosomes of patients with intractable GD may activate immune cells, which may play an important role in GD pathogenesis.

PMID: 27610819 [PubMed - as supplied by publisher]

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