Abstract
The purpose of this study was to examine extra cellular matrix composition, vascularization and immune cell population of skin sites prone to keloid formation.
Keloids remain a complex problem, posing esthetical as well as functional difficulties for those affected. These scars tend to develop at anatomic sites of preference. Mechanical properties of skin vary with anatomic location and depend largely on extra cellular matrix composition. These differences in extra cellular matrix composition, but also vascularization and resident immune cell populations might play a role in the mechanism of keloid formation.
To examine this hypothesis, skin samples of several anatomic locations were taken from 24 human donors within zero to 36 hours after they had deceased. Collagen content and cross-links were determined through high-performance liquid chromatography. The expression of several genes, involved in extra cellular matrix production and degradation, was measured by means of real-time PCR. (Immuno) histochemistry was performed to detect fibroblasts, collagen, elastin, blood vessels, Langerhans cells and macrophages. Properties of skin of keloid predilections sites were compared to properties of skin from other locations (non-predilection sites).
The results indicated that there are site specific variations in extracellular matrix properties (collagen and cross-links) as well as macrophage numbers. Moreover, predilection sites for keloid formation contain larger amounts of collagen compared to non-predilection sites, but decreased numbers of macrophages, in particular classically activated CD40 positive macrophages.
In conclusion, the altered (histological, protein and genetic) properties of skin of keloid predilection sites may cause a predisposition for and contribute to keloid formation. This article is protected by copyright. All rights reserved.
from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2iDOg9d
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