Calcium-regulated chloride channel anoctamin-1 is present in the suprachiasmatic nuclei of rats

Calcium-regulated chloride channel (CaCC) anoctamin-1 has been recently identified in neurons. In neurons, which express the Na+–K+–2Cl− cotransporter, activation of CaCCs increases firing frequency, by reversion between the Cl− equilibrium potential and the membrane resting potential, leading to membrane depolarization by Cl− extrusion from the cell. Although there are no reports of CaCCs present in the suprachiasmatic nuclei (SCN), the fact that Na+–K+–2Cl− cotransporter is present in SCN neurons, where it has been shown to be involved in the excitatory effects of γ-aminobutyric acid, together with the increase of neuronal firing rate induced by release of intracellular Ca2+ after administration of 100 nM ryanodine, leads us to determine whether CaCCs are present in the SCN. Immunohistochemistry and western blots show the expression of the CaCCs anoctamin-1 protein. Quantitative PCR demonstrated the expression of anoctamin-1 mRNA in the SCN. These results clearly indicate the presence of CaCC in SCN of rats. Correspondence to Raúl Aguilar-Roblero, MD, PhD, Departamento de Neurociencias Cognitivas, División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, PO Box 70-253, Mexico City 04510, Mexico Tel: +52 55 5622 5650; fax: +52 55 5622 5607; e-mail: raguilar@ifc.unam.mx Received October 27, 2017 Accepted October 31, 2017 © 2018 Wolters Kluwer Health | Lippincott Williams & Wilkins

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