miR-150-5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR

xlomafota13 shared this article with you from Inoreader miR-150-5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR Via Experimental and therapeutic medicine Exp Ther Med. 2022 Apr;23(4):283. doi: 10.3892/etm.2022.11213. Epub 2022 Feb 15. ABSTRACT Dysregulated microRNAs (miRNAs or miRs) serve potential roles in inflammatory systemic disease, including ankylosing spondylitis (AS). The aim of the present study was to investigate the potential function of miR-150-5p in osteogenic differentiation of AS fibroblasts and its underlying mechanism. The expression of miR-150-5p and vitamin D receptor (VDR) in AS joint capsules and fibroblasts was detected by reverse transcription-quantitative (RT-q)PCR and western blotting. Following overexpression of miR-150-5p, the alteration in osteogenic gene expression was detected by RT-qPCR, western blotting and alkaline phosphatase activity assay, as well as alizarin red staining. The association between miR-150-5p and VDR was confirmed by luciferase assay and rescue experiments were performed. Patients with AS exhibited decreased expression of miR-150-5p in joint capsules. Treatment with bone morphogenic protein 2 (BMP-2) and transforming growth factor-β1 (TGF-β1) led to downregulation of miR-150-5p in AS fibroblasts. Enforced expression of miR-150-5p attenuated osteogenic differentiation of AS fibroblasts. These results demonstrated that miR-150-5p inhibited osteogenic differentiation of AS fibroblasts by targeting VDR. miR-150-5p overexpression decreased osteogenic transformation of fibroblasts by decreasing VDR expression in AS. PMID:35317439 | PMC:PMC8908459 | DOI:10.3892/etm.2022.11213 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Circular RNA hsa_circ_0011946 promotes the malignant process of salivary adenoid cystic carcinoma by downregulating miR-1205 expression

xlomafota13 shared this article with you from Inoreader Circular RNA hsa_circ_0011946 promotes the malignant process of salivary adenoid cystic carcinoma by downregulating miR-1205 expression Via Experimental and therapeutic medicine Exp Ther Med. 2022 Apr;23(4):295. doi: 10.3892/etm.2022.11224. Epub 2022 Feb 18. ABSTRACT Circular RNA (circRNA/circ) hsa_circ_0011946 has been reported to serve an important role in a number of cancer types; however, to the best of our knowledge, its role in salivary adenoid cystic carcinoma (SACC) has not been reported. In the present study, the primary focus was the effects of hsa_circ_0011946 on the invasion, migration and epithelial-mesenchymal transformation (EMT) of SACC cells, and the specific mechanisms involved. The expression levels of hsa_circ_0011946 and microRNA (miR)-1205 in cancer tissues and paracancerous tissues of patients with SACC were analyzed using reverse transcription-quantitative (RT-q)PCR. The cell proliferation rate was determined using a Cell Counting Kit-8 assay. Wound healing assays were performed to analyze the cell migratory ability, while a transwell assay was used to measure the cell invasion ability. Western blotting was used to analyze the expression levels of EMT-related proteins. Cell transfection was used to knockdown hsa_circ_0011946 and knockdown or overexpress miR-1205. Subcellular localization assays for hsa_circ_0011946 were performed using RT-qPCR. A dual-luciferase reporter gene assay was used to verify the binding between hsa_circ_0011946 and miR-1205. The results of the present study revealed that the expression levels of hsa_circ_0011946 were significantly upregulated in cancer tissues from patients with SACC. The knockdown of hsa_circ_0011946 expression inhibited the proliferation, invasion and migration of SACC cells, thereby inhibiting the EMT process, which was achieved by downregulating miR-1205 expression. In conclusion, circRNA hsa_circ_0011946 was discovered to promote the malignant process of SACC by downregulating miR-1205 expression. PMID:35317442 | PMC:PMC8908474 | DOI:10.3892/etm.2022.11224 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

pH regulates the lumen diameter of tissue-engineered capillaries

xlomafota13 shared this article with you from Inoreader pH regulates the lumen diameter of tissue-engineered capillaries Via Experimental and therapeutic medicine Exp Ther Med. 2022 Apr;23(4):284. doi: 10.3892/etm.2022.11212. Epub 2022 Feb 15. ABSTRACT Angiogenesis is vital in tissue engineering and the size of the capillary lumen diameter directly affects vascular function. Therefore, the involvement of the pH in the regulation of the capillary lumen diameter was investigated in the present study. The cytosolic pH of different pH medium groups was measured using flow cytometry. Bromodeoxyuridine staining and wound-healing assays were performed to detect cell proliferation and migration, respectively. The expression of angiogenesis-related genes was detected using reverse transcription-quantitative PCR. In addition, cell tube formation under different pH conditions was assessed using a tube formation assay and a 3D Matrigel® model. The results indicated that a change in the pH value of the culture medium affected the cytosolic pH of the endothelial cells, which then led to a change i n vascular diameter. When the medium's pH ranged from 7.4 to 7.6, the diameter of the lumen formed in the Matrigel was suitable for capillary formation in tissue engineering. The present results revealed an important role for the pH in the process of capillary formation and provided insight for pH regulation during endothelial cell tube formation and angiogenesis in tissue engineering. PMID:35317437 | PMC:PMC8908470 | DOI:10.3892/etm.2022.11212 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Neuroradiological Basis of COVID‐19 Olfactory Dysfunction: A Systematic Review and Meta‐Analysis

xlomafota13 shared this article with you from Inoreader Neuroradiological Basis of COVID‐19 Olfactory Dysfunction: A Systematic Review and Meta‐Analysis Via The Laryngoscope Objective Olfactory dysfunction (OD) is a common presenting symptom of COVID-19 infection. Radiological imaging of the olfactory structures in patients with COVID-19 and OD can potentially shed light on its pathogenesis, and guide clinicians in prognostication and intervention. Methods PubMed, Embase, Cochrane, SCOPUS were searched from inception to August 1, 2021. Three reviewers selected observational studies, case series, and case reports reporting radiological changes in the olfactory structures, detected on magnetic resonance imaging, computed tomography, or other imaging modalities, in patients aged ≥18 years with COVID-19 infection and OD, following preferred reporting items for systematic reviews and meta-analyses guidelines and a PROSPERO-registered protocol (CRD42021275211). We described the proportion of radiological outcomes, and used random-effects meta-analyses to pool the prevalence of olfactory cleft opacification, olfactory bulb signal abnormalities, and olfactory mucosa abnormalities in patients with and without COVID-19-associated OD. Results We included 7 case–control studies (N = 353), 11 case series (N = 154), and 12 case reports (N = 12). The pooled prevalence of olfactory cleft opacification in patients with COVID-19 infection and OD (63%, 95% CI = 0.38–0.82) was significantly higher than that in controls (4%, 95% CI = 0.01–0.13). Conversely, similar proportions of cases and controls demonstrated olfactory bulb signal abnormalities (88% and 94%) and olfactory mucosa abnormalities (2% and 0%). Descriptive analysis found that 55.6% and 43.5% of patients with COVID-19 infection and OD had morphological abnormalities of the olfactory bulb and olfactory nerve, respectively, while 60.0% had abnormal olfactory bulb volumes. Conclusion Our findings implicate a conductive mechanism of OD, localized to the olfactory cleft, in approximately half of the affected COVID-19 patients. Laryngoscope, 2022 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Radiologic–pathologic correlation of major versus minor extranodal extension in oral cavity cancer

xlomafota13 shared this article with you from Inoreader Radiologic–pathologic correlation of major versus minor extranodal extension in oral cavity cancer Via Head & Neck Abstract Background To evaluate the diagnostic performance of radiologic extranodal extension (rENE) in predicting major (>2 mm) and minor (≤2 mm) pathologic ENE (pENE). Methods All oral cavity squamous cell carcinoma patients who underwent neck dissection with pathological nodal disease (pN+) between 2010 and 2015 were reviewed. Preoperative computed tomography and/or magnetic resonance imaging were reviewed by two head and neck neuroradiologists. Results Three hundred and thirty-four patients were included. The sensitivity and specificity of rENE were 37% [95% CI 29–44] and 98% [95% CI 96–100], respectively. Sensitivity for pENE improved in the subset of patients with major ENE (48% [95% CI 38–57]). The presence of rENE was associated with inferior 3-year overall survival: 26% [95% CI 17–41] versus 60% [95% CI 54–67]. Conclusions This large cohort study demonstrates high specificity, but low sensitivity for preoperative imaging in the detection of pENE in OCSCC. Patients with rENE demonstrated poor OS. pENE in the absence of rENE is still an adverse risk factor. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Second primary malignancies induced by radioactive iodine treatment of differentiated thyroid carcinoma - a critical review and evaluation of the existing evidence radioactive iodine 5h Tracking Uptake and Metabolism of Xenometallomycins Using a Multi-Isotope Tagging Strategy radioactive iodine 5h A Study to Assess the Effect of Size & Site of Tympanic Membrane Perforation on Hearing Loss Otolaryngology Indian 8h Response to “Radioiodine Is Molecular Radiotherapy Governed by Predictable Deterministic Radiobiology Expressed in Gray, Not Millicuries” by Kao liebertpub1 12h Sex Differences in Differentiated Thyroid Cancer liebertpub1 12h Unique Molecular Signatures Are Associated with Aggressive Histology in Pediatric Differentiated Thyroid Cancer liebertpub1 12h Severe Resistance to Thyroid Hormone Beta in a Patient with Athyreosis liebertpub1 12h Follicular and Hurthle Cell Carcinoma: Comparison of Clinicopathological Features and Clinical Outcomes liebertpub1 12h For more inform

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5h
Tracking Uptake and Metabolism of Xenometallomycins Using a Multi-Isotope Tagging Strategy
radioactive iodine
5h
A Study to Assess the Effect of Size & Site of Tympanic Membrane Perforation on Hearing Loss
Otolaryngology Indian
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Response to "Radioiodine Is Molecular Radiotherapy Governed by Predictable Deterministic Radiobiology Expressed in Gray, Not Millicuries" by Kao
liebertpub1
12h
Sex Differences in Differentiated Thyroid Cancer
liebertpub1
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Unique Molecular Signatures Are Associated with Aggressive Histology in Pediatric Differentiated Thyroid Cancer
liebertpub1
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Severe Resistance to Thyroid Hormone Beta in a Patient with Athyreosis
liebertpub1
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Follicular and Hurthle Cell Carcinoma: Comparison of Clinicopathological Features and Clinical Outcomes
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Enhancing Radioiodine Incorporation in BRAF-Mutant, Radioiodine-Refractory Thyroid Cancers with Vemurafenib and the Anti-ErbB3 Monoclonal Antibody CDX-3379: Results of a Pilot Clinical Trial
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Comparison and Validation of Acoustic Voice Quality Index Version 2 and Version 3 among South Indian Population
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Where do we come from? Where are we? Where are we going? : President's speech at the 92nd Annual Meeting of the German Society of Otorhinolaryngology, Head and Neck Surgery on the occasion of its centennial. German version
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Monomeric C-reactive protein level is associated with osteoarthritis
Experimental and therapeutic medicine
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MicroRNA-302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1
Experimental and therapeutic medicine
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miR-150-5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR
Experimental and therapeutic medicine
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Expression of TMED3 is independently associated with colorectal cancer prognosis
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Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia-induced pancreatitis <em>in vitro</em>
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Micropulse vs. continuous wave transscleral cyclophotocoagulation in neovascular glaucoma
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Effects and early diagnostic value of lncRNA H19 on sepsis-induced acute lung injury
Experimental and therapeutic medicine
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Adrenocortical carcinoma: Pediatric aspects (Review)
Experimental and therapeutic medicine
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Circular RNA hsa_circ_0011946 promotes the malignant process of salivary adenoid cystic carcinoma by downregulating miR-1205 expression
Experimental and therapeutic medicine
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MicroRNA-200a-3p and GATA6 are abnormally expressed in patients with non-small cell lung cancer and exhibit high clinical diagnostic efficacy
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Long non-coding RNA TUG1 promotes osteosarcoma cell proliferation and invasion through inhibition of microRNA-212-3p expression
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Nephroprotective effect of magnesium isoglycyrrhizinate against arsenic trioxide-induced acute kidney damage in mice
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Anagliptin promotes apoptosis in mouse colon carcinoma cells via MCT-4/lactate-mediated intracellular acidosis
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pH regulates the lumen diameter of tissue-engineered capillaries
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Diabetes mellitus: Plasticizers and nanomaterials acting as endocrine-disrupting chemicals (Review)
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S100A9 blockade improves the functional recovery after spinal cord injury via mediating neutrophil infiltration
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Effectiveness of gabapentin in reducing opioid requirements after radiation in head and neck cancer in a single institution
Head & Neck
20h
Radiologic–pathologic correlation of major versus minor extranodal extension in oral cavity cancer
Head & Neck

Effectiveness of gabapentin in reducing opioid requirements after radiation in head and neck cancer in a single institution
20h
by
David R. Gilley, Andrew D. Clark, Maggie E. Wieser, Craig A. Bollig, Laura M. Dooley, Gregory B. Biedermann
via
Head & Neck
Abstract
Background
Gabapentin has been shown to reduce opioid use in head and neck cancer patients. Here, we examine the efficacy of prophylactic gabapentin at reducing opioid use in these patients at our institution.

Methods
A retrospective study of patients receiving radiation was performed, using patients from our previous study as controls. Risk factors for opioid use at 3 and 6 months were determined using univariate and multivariate analyses.

Results
In total, 53/185 patients were treated with gabapentin, 39.6% of which took opioids at 3 months vs. 58.3% in the non-gabapentin cohort (p = 0.021). Gabapentin was independently associated with less opioid use on multivariate analysis at 3 months (OR 0.47, 95% CI 0.24–0.9). Gastrostomy tube dependence and pretreatment opioid use were associated with chronic opioid use despite gabapentin.

Conclusions
Gabapentin is effective at expediting opioid tapering in head and neck cancer patients who are not gastrostomy tube dependent or taking opioids pretreatment.

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Radiologic–pathologic correlation of major versus minor extranodal extension in oral cavity cancer
20h
by
Michael A. Blasco, Christopher W. Noel, Tra Truong, Shao Hui Huang, David P. Goldstein, Jonathan C. Irish, Ralph Gilbert, Ali Hosni, Andrew Hope, Brian O'Sullivan, John Waldron, Bayardo Perez‐Ordonez, Ilan Weinreb, Stephen M. Smith, Eric Bartlett, Eugene Yu, John R. Almeida
via
Head & Neck
Abstract
Background
To evaluate the diagnostic performance of radiologic extranodal extension (rENE) in predicting major (>2 mm) and minor (≤2 mm) pathologic ENE (pENE).

Methods
All oral cavity squamous cell carcinoma patients who underwent neck dissection with pathological nodal disease (pN+) between 2010 and 2015 were reviewed. Preoperative computed tomography and/or magnetic resonance imaging were reviewed by two head and neck neuroradiologists.

Results
Three hundred and thirty-four patients were included. The sensitivity and specificity of rENE were 37% [95% CI 29–44] and 98% [95% CI 96–100], respectively. Sensitivity for pENE improved in the subset of patients with major ENE (48% [95% CI 38–57]). The presence of rENE was associated with inferior 3-year overall survival: 26% [95% CI 17–41] versus 60% [95% CI 54–67].

Conclusions
This large cohort study demonstrates high specificity, but low sensitivity for preoperative imaging in the detection of pENE in OCSCC. Patients with rENE demonstrated poor OS. pENE in the absence of rENE is still an adverse risk factor.

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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,

Telephone consultation 11855 int 1193,

Neue Therapieansätze für Speicheldrüsenmalignome

xlomafota13 shared this article with you from Inoreader Neue Therapieansätze für Speicheldrüsenmalignome Via Laryngo-Rhino-Otologie Laryngorhinootologie DOI: 10.1055/a-1792-2246 In den vergangenen Jahren haben sich durch die Verbesserung und Verbreitung molekularpathologischer Testverfahren und die Möglichkeit von zielgerichteten Therapien neue Optionen in der Behandlung von Speicheldrüsenmalignomen ergeben. Durch die neuen zielgerichteten Therapieoptionen werden Patienten deutlich weniger toxische Alternativen zur konventionellen zytostatischen Chemotherapie geboten. Zum einen gibt es neue entitätsspezifische Therapien, wie z.B. die Therapie mit NTRK-Inhibitoren bei sekretorischen Karzinomen und die Therapie mit Axitinib bei adenoidzystischen Karzinomen. Darüber hinaus rücken aber auch entitätsübergreifende Therapeutika, wie z.B. die antiandrogene The rapie, die HER2-Inhibition oder auch die PI3K-Inhibition, in den Vordergrund. Bei metastasierten/rezidivierten Speicheldrüsenkarzinomen, welche nicht mit einer zielgerichteten Therapie behandelt werden können, werden weiterhin hauptsächlich platinbasierte Chemotherapien eingesetzt. [...] Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, Germany Article in Thieme eJournals: Table of contents  |  Abstract  |  Full text View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Endoscopic dacryocystorhinostomy for refractory nasolacrimal duct obstruction with a small lacrimal sac (≤ 5 mm in diameter)

xlomafota13 shared this article with you from Inoreader Endoscopic dacryocystorhinostomy for refractory nasolacrimal duct obstruction with a small lacrimal sac (≤ 5 mm in diameter) Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2022 Mar 23. doi: 10.1007/s00405-022-07347-1. Online ahead of print. ABSTRACT BACKGROUND: Although endoscopic dacryocystorhinostomy (DCR) is a standard procedure for nasolacrimal duct obstruction (NLDO), the failure rate remains approximately 10%. A small lacrimal sac is considered the main reason for surgical failure. We explored the efficacy of endoscopic DCR for the treatment of NLDO with a small lacrimal sac. METHODS: The clinical data of 72 patients (88 eyes) diagnosed with NLDO and undergoing endoscopic DCR from 2012 to 2020, with at least 24 months of follow-up were retrospectively collected. Intraoperatively, the Rosenmüller valves were fully exposed, mucosal flaps were preserved to cover the naked bone, and a silicone tube was implanted if necessary. Postoperative intervention was performed if necessary. The main outcome measures were symptomatic improvement and objective ostium patency. RESULTS: Eighty-eight eyes of 72 patients were divided into two groups: the refractory group (34 patients, 47 eyes), with a small lacrimal sac (≤ 5 mm in diameter), and the simple group (38 patients, 41 eyes). Patients with small lacrimal sacs were more prone to bilateral eye disease than those in the simple group (P = 0.014) and required a longer postoperative follow-up (P < 0.001). Refractory NLDO and simple NLDO had a success rate of 91.5% and 95.1%, respectively, with no significant difference. CONCLUSION: Endoscopic DCR for refractory NLDO with a small lacrimal sac could achieve a beneficial result by exposing the Rosenmüller valves, preserving mucosal flaps, implanting necessary intubation, and intervening postoperatively. Thus, a small lacrimal sac should not be regarded as a contraindication to surgery. PMID:35320400 | DOI:10.1007/s00405-022-07347-1 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Pelvic Pain after italian coffee consumption : Ischemic colitis (IC) is an inflammatory condition of the large intestine, or colon. It develops when there isn't enough blood flow to the colon.

Pelvic pain occurs mostly in the lower abdomen area. The pain might be steady, or it might come and go. It can be a sharp and stabbing pain in a specific spot, or a dull pain that is spread out. If the pain is severe, it might get in the way of your daily activities.Pelvic pain can be steady, or it can come and go. It can be a sharp and stabbing pain felt in a specific spot, or a dull pain that is spread out.

Ischemic colitis occurs when blood flow to part of the large intestine (colon) is temporarily reduced, usually due to constriction of the blood vessels supplying the colon or lower flow of blood through the vessels due to low pressures.

For patients who present with symptoms of ischemia, a CT scan of the abdomen with oral and IV contrast and laboratory testing can be performed. Manifestations of ischemic colitis on CT include colonic wall thickening, edema, thumbprinting, bowel dilatation, and effusion of intestinal circumference.

  

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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,

Telephone consultation 11855 int 1193,