The Gas Sampling Interval Effect on V˙O2peak Is Independent of Exercise Protocol

Introduction: There is a plethora of gas sampling intervals available during cardiopulmonary exercise testing to measure peak oxygen consumption (V˙O2peak). Different intervals can lead to altered V˙O2peak. Whether differences are affected by the exercise protocol or subject sample is not clear. The purpose of this investigation was to determine whether V˙O2peak differed because of the manipulation of sampling intervals and whether differences were independent of the protocol and subject sample. Methods: The first subject sample (24 ± 3 yr; V˙O2peak via 15-breath moving averages: 56.2 ± 6.8 mL·kg−1·min−1) completed the Bruce and the self-paced V˙O2max protocols. The second subject sample (21.9 ± 2.7 yr; V˙O2peak via 15-breath moving averages: 54.2 ± 8.0 mL·kg−1·min−1) completed the Bruce and the modified Astrand protocols. V˙O2peak was identified using five sampling intervals: 15-s block averages, 30-s block averages, 15-breath block averages, 15-breath moving averages, and 30-s block averages aligned to the end of exercise. Differences in V˙O2peak between intervals were determined using repeated-measures ANOVAs. The influence of subject sample on the sampling effect was determined using independent t-tests. Results: There was a significant main effect of sampling interval on V˙O2peak (first sample Bruce and self-paced V˙O2maxP

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