The liver X receptors (LXRs) is an important component of the nuclear receptor (NR) superfamily. Previous studies have shown that the LXRs possessed antitumor activity in various types of tumor cells. However, the complicated mechanisms underlying the antitumor activity remain largely unexplored. In this study, we incubated A549 cells with the compound T0901317, a specific LXRs agonist, for 24 h. The MTT assay was used to assess cell viability. Transwell assays were used to evaluate cell migration and invasion. The shRNA was utilized for RNA interference. The target gene and protein expression levels were assessed using reverse transcription-PCR and western blot assay. The DNA-binding activity of nuclear factor κB (NF-κB) was examined using electrophoretic mobility shift assays. Luciferase reporter assay was used to detect the binding of NF-κB to the matrix metalloproteinase-9 (MMP-9) promoter. We found that T0901317 inhibited the invasion and migration of A549 cells in a dose-dependent manner. Meanwhile, we further indicated that activation of LXRβ, one subtype of LXRs, can downregulate MMP-9 expression. More importantly, activation of LXRβ triggered by T0901317 inhibited the invasion and metastasis of A549 cells by repressing NF-κB/MMP-9 signaling pathway. Taken together, our study shows that activation of LXRs triggered by T0901317 inhibits the invasion and metastasis of human non-small-cell lung cancer by repressing the NF-κB/MMP-9 signaling pathway.
from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2fcoMhW
Αρχειοθήκη ιστολογίου
-
►
2023
(269)
- ► Φεβρουαρίου (133)
- ► Ιανουαρίου (136)
-
►
2022
(2046)
- ► Δεκεμβρίου (165)
- ► Σεπτεμβρίου (161)
- ► Φεβρουαρίου (165)
-
►
2021
(3028)
- ► Δεκεμβρίου (135)
- ► Σεπτεμβρίου (182)
- ► Φεβρουαρίου (324)
-
►
2020
(1051)
- ► Δεκεμβρίου (292)
- ► Σεπτεμβρίου (60)
- ► Φεβρουαρίου (28)
-
►
2019
(2277)
- ► Δεκεμβρίου (18)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (89)
-
►
2018
(26280)
- ► Δεκεμβρίου (189)
- ► Φεβρουαρίου (6130)
- ► Ιανουαρίου (7050)
-
▼
2017
(33948)
- ► Δεκεμβρίου (6715)
-
▼
Σεπτεμβρίου
(6470)
-
▼
Σεπ 16
(133)
- Mouse Model of IL-17-Dominant Rhinitis Using Polyi...
- A Retrospective Study of Korean Adults With Food A...
- Effects of Immunoglobulin Replacement on Asthma Ex...
- Seasonal Cycle and Relationship of Seasonal Rhino-...
- Association Between Sensitization to Mold and Impa...
- Patterns of Inhalant Allergen Sensitization and Ge...
- Prevalence of Respiratory Viral Infections in Kore...
- Clinical Characteristics of Exacerbation-Prone Adu...
- Chronic Spontaneous Urticaria: Pathogenesis and Tr...
- Asthma Biomarkers: Do They Bring Precision Medicin...
- Effective Strategies for Managing Asthma Exacerbat...
- Mirror trends of plasticity and stability indicato...
- Frequency-dependent regulation of intrinsic excita...
- Altered Cav1.2 function in the Timothy Syndrome mo...
- Inflammation alters AMPA-stimulated calcium respon...
- FoxP expression identifies a Kenyon cell subtype i...
- Three shades of grey: detecting brain abnormalitie...
- The Neurobiological Bases of Autism Spectrum Disor...
- Effects of the availability of accurate propriocep...
- Long-Range Cortical Dynamics: A Perspective from t...
- Does an observer's empathy influence my pain? Effe...
- Processing of temporally patterned sounds in the a...
- Modulating vicarious tactile perception with trans...
- Temporal alignment of anticipatory motor cortical ...
- Encoding seasonal information in a two-oscillator ...
- AllergoOncology: Opposite Outcomes of Immune Toler...
- Tonsillectomy in adults: Analysis of indications a...
- TLR expression profile of human alveolar bone prop...
- A novel method to prevent catheter dysfunction dur...
- Accidental fracture of an EUS-FNA needle
- Use of Volumetric Laser Endomicroscopy to Characte...
- Three-dimensional strut plate for the treatment of...
- Oropharyngeal epignathus with partial facial dupli...
- Dendritic spines provide cognitive resilience agai...
- Cancer-specific mortality of high-risk prostate ca...
- The miR-203 inhibits cell proliferation, invasion,...
- Implementation and preliminary clinical outcomes o...
- HLA class I expression predicts prognosis and ther...
- Effects of tetramethylpyrazine phosphate on pancre...
- HLA class I expression predicts prognosis and ther...
- A retrospective review of initial bisphosphonate i...
- Evaluation of neurodevelopment of children with co...
- HLA class I expression predicts prognosis and ther...
- In vitro inhibition of HIV-1 replication in autolo...
- Effects of tetramethylpyrazine phosphate on pancre...
- Conventional as well as Emerging Arsenic Removal T...
- Concurrent Improvement in Both Binge Eating and De...
- Notch signaling pathway networks in cancer metasta...
- Skeletal muscle mechanics: questions, problems and...
- Rare Giant Upper Lip Epidermal Cyst in a Patient W...
- Dosimetric comparison of different treatment modal...
- "Gan To Kagaku Ryoho"[jour]; +20 new citations
- Complications and parent satisfaction in pediatric...
- Is hippocampal neurogenesis modulated by the sensa...
- Is hippocampal neurogenesis modulated by the sensa...
- Health-related quality of life in melanoma patient...
- Isolated limb perfusion for locally advanced angio...
- [Clinical study of aged patients with secondary be...
- PDL1 And LDHA act as ceRNAs in triple negative bre...
- Overview of Sinonasal and Ventral Skull Base Malig...
- [Clinical analysis of orbital complications due to...
- Scaling of dentition and prey size in the Californ...
- Adding Expansile Duraplasty to Posterior Fossa Dec...
- Posterior Fossa Craniectomy with Endovascular Ther...
- A Hebrew Adaptation of the Tinnitus Functional Index.
- What’s Next in Complex Networks? Capturing the Con...
- Understanding the Science of Resistance Training: ...
- Neoisoliquiritigenin inhibits tumor progression by...
- Mild to Moderate Dysphagia Following Very Low-dose...
- Adult acute supraglottitis: Analysis of 358 patien...
- NR4A1 is an endogenous inhibitor of vocal fold fib...
- Effects of voice-sparing cricotracheal resection o...
- Giant epiglottic cyst: Is tracheostomy essential?
- Laryngoscopic and stroboscopic signs in the diagno...
- Time-driven activity-based costing to estimate cos...
- Phosphorylated FADD is not prognostic for local co...
- Interleukin-1β and interleukin-6 enhance thermal p...
- SMAD3 expression and regulation of fibroplasia in ...
- Upper aerodigestive tract cancer and oral health s...
- Minimally Invasive Laparoscopic Dissected - Deep I...
- Bilateral dystrophic ossification of the thyroid c...
- Concomitant diseases and their effect on disease p...
- Honest signaling in domestic piglets (Sus scrofa d...
- "Gan To Kagaku Ryoho"[jour]; +20 new citations
- The relationship between the GJB3 c.538C>T variant...
- Utility of screening questionnaire and polysomnogr...
- Effects of pregabalin on postoperative pain after ...
- Contralateral Breast Cancers: Independent Cancers ...
- The impact of P-glycoprotein and Breast Cancer Res...
- Neuroblastoma cells undergo transcriptomic alterat...
- Ingested nitrate and nitrite, disinfection by-prod...
- A naive Bayes algorithm for tissue origin diagnosi...
- Tumor-suppressive miR-145 co-repressed by TCF4-β-c...
- Dacomitinib-induced diarrhea: Targeting chloride s...
- Associations of alcohol intake, smoking, physical ...
- Erratum to “The “Crumple Zone” hypothesis: Associa...
- Exercise prescription for patients with multiple s...
- Flap repair of complex pilonidal sinus: a single i...
- New Survey Shows Growing Openness about Hearing Loss
- Biomarker levels in gingival crevicular fluid of g...
-
▼
Σεπ 16
(133)
-
►
2016
(4179)
- ► Σεπτεμβρίου (638)
- ► Φεβρουαρίου (526)
- ► Ιανουαρίου (517)
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου