T cells are involve in the induction of macrophage phenotypes in oral leukoplakia and squamous cell carcinoma – a preliminary report

Abstract

Background

The prognosis of human malignancies has been shown to depend on immunological parameters, such as macrophage polarisation (M1 and M2). In the present study, we identify the phenotype of macrophages, and investigate an involvement of infiltrated T cells that participate in the polarization of macrophages, in oral leukoplakia (OLK), and oral squamous cell carcinoma (OSCC).

Methods

Immunohistochemical method was used to examine the number of CD68⁺, CD163⁺ (M2), iNOS⁺ (M1) macrophages, and CD4⁺, CD8⁺, CCR4⁺ (Th2), CCR5⁺ (Th1) cells in 102 cases of OSCC: without metastases – OSCC M(-) (n=54), and with metastases – OSCC M(+) (n=48), 23 cases of OLK, and 18 control cases.

Results

The mean number of CD68⁺, CD163⁺, iNOS⁺, CD4⁺, CCR4⁺, CCR5⁺ cells was significantly increased in OSCC M(+) group compared with OLK, OSCC M(-) and control group. We found positive correlations between the number of CD4⁺ T cells and CD163⁺ and iNOS⁺ macrophages as well as CCR4⁺ and CCR5⁺ cells in both OSCC groups. The mean number of CD8⁺ cells was significantly increased in OSCC M(-) and OLK compared with OSCC M(+) and control group. In OSCC M(+) and OSCC M(-) groups a negative correlation between the number of CD8⁺ cells and CD163⁺ and iNOS⁺ macrophages was found.

Conclusions

The number and co-localization of lymphocytes and macrophages in OLK and OSCC, may indicate that infiltrating cells influence the early and subsequent stage of oral carcinogenesis.

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from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2zo7GUc