Oncogenic viruses in Skull Base Chordomas.

Oncogenic viruses in Skull Base Chordomas.

World Neurosurg. 2017 Nov 27;:

Authors: Yakkioui Y, Speel EM, Van Overbeeke JJ, Boderie MJM, Pujari S, Hausen AZ, Wolffs PFG, Temel Y

Abstract
BACKGROUND: Chordomas are rare tumors assumed to derive from notochordal remnants. We believe that a molecular switch is responsible for their malignant behaviour. However, the involvement of oncogenic viruses has not been studied. Thus, in the present study we investigate the presence of oncogenic viruses in chordomas.
METHODS: DNA and RNA from 18 snap-frozen chordomas and 15 chondrosarcomas were isolated. Real-time PCR or RT-PCR was performed to assess the presence of multiple oncogenic viruses, including herpesviridea (HSV1, HSV2,EBV, CMV, HHV6, HHV7 and KSHV), polyomaviridea (PVB19, BK, JC, SV40, MCPyV, HPyV6 and HPyV7), papillomaviridae and respiratory viruses. Immunohistochemistry (IHC) and in situ hybridization (ISH) were used to validate the positive results.
RESULTS: PVB19 DNA was detected in 4/18 (22%) and in 1/15 (7%) of the chordomas and chondrosarcomas respectively. IHC recognizing the VP2 capsid protein of PVB19 showed a positive cytoplasmic staining in 44% (14/32) of the cases. HHV7 DNA was present in 6 out of 18 (33%) chordomas. Genomic DNA of EBV was found in 22% of samples. However, no positive result was found in the ISH. None of the chordoma cases showed any presence of DNA from the remaining viruses.
CONCLUSION: Viral involvement in the etiology of chordomas is likely, with PVB19 being the most distinguishing.

PMID: 29191533 [PubMed - as supplied by publisher]

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