Radioiodination and Purification of [131I]β-CIT and [131I]FP-CIT with an Automated Radiosynthesizer

xlomafota13 shared this article with you from Inoreader Radioiodination and Purification of [<sup>131</sup>I]β-CIT and [<sup>131</sup>I]FP-CIT with an Automated Radiosynthesizer Via radioactive iodine Pharmaceuticals (Basel). 2022 Jan 14;15(1):96. doi: 10.3390/ph15010096. ABSTRACT Dopaminergic transporter (DAT) imaging with single photon emission computed tomography (SPECT) is used to diagnose Parkinson's disease and to differentiate it from other neurodegenerative disorders without presynaptic dopaminergic dysfunction. The radioiodinated tropane alkaloids [123I]FP-CIT and [123I]β-CIT enable the evaluation of the integrity of DATs. Commonly, the labeling o f these compounds is performed by electrophilic substitution of the alkylstannylated precursors with radioactive iodine and following purification by HPLC or solid phase extraction (SPE). This work presents the first radioiodination of β-CIT and FP-CIT with no carrier added [131I]NaI on a Scintomics GRP synthesis module. Free iodine-131 and impurities were removed by SPE over a C-18 Sep-Pak cartridge. We achieved a radiochemical yield of >75% and a radiochemical purity of >98% with both compounds. Our development of an automated synthesis on a commercially available synthesizer ensures robust and efficient labeling of [131I]FP-CIT and [131I]β-CIT starting with low concentrated radioiodine. PMID:35056152 | DOI:10.3390/ph15010096 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.