Parathyroid hormone-related peptide (1–34) reduces alveolar bone loss in type 1 diabetic rats

Publication date: November 2017
Source:Archives of Oral Biology, Volume 83
Author(s): Wen Zhang, Su-Zhen Wu, Jian Zhou, Hong-Min Chen, Ye-Li Gong, Fang-Fang Peng, Bai-Fang Zhang
ObjectiveTo investigate the role of parathyroid hormone related protein (PTHrP) in diabetic periodontitis.MethodsAfter injected with 55mg/kg streptozotocin, diabetic rats were treated subcutaneously with low-dose (40μg/kg, once daily for 5days per week), middle-dose (80μg/kg) or high-dose (160μg/kg) PTHrP(1–34) peptide. Treatment continued for 12 weeks. Changes in periodontal tissues were confirmed by micro-computerized tomography assay and H&E analysis. We used tartrate resistant acid phosphatase (TRAP) staining to identify osteoclast cells. The expression of TNF-α, IL-1β and IL-6 was assessed by immunohistochemistry and Western blot.ResultsTooth-supporting structure loss was observed in periodontal tissues of diabetic rats. PTHrP (1–34) attenuated alveolar bone loss, especially in the middle-dose and high-dose group. Whereas TNF-α, IL-1β and IL-6 protein levels were increased in the diabetic gingival tissues, PTHrP (1–34) treatment inhibited the increase of IL-1β and IL-6, but had no effect on TNF-α.ConclusionType 1 diabetes increased the susceptibility to periodontal disease. Intermittent administration of PTHrP (1–34) exhibited an inhibitory effect on alveolar bone resorption and the gingival inflammation in periodontal tissues of diabetic rats.



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