Abstract
Background
Cellular motility is considered central to the process of metastasis and podoplanin expression can be explored as a prospective link due to its ability to modulate actin cytoskeleton. We aimed to evaluate the tumoural expression of D2-40 (mono-clonal antibody to podoplanin) in pathologically neck-node negative/positive cases (pN0/N+) to characterise the pattern of invasion, potentially explaining the role of various patterns of invasion in causing tumor metastasis.
Methods
Paraffin-embedded tissue blocks of 60 cases of OSCC of known nodal status were selected for immunohistochemical staining of tumour (invasive front) by D2-40 (anti-podoplanin) along with routine staining by Haematoxylin & Eosin. Various staining patterns were assessed and evaluated for expression of D2-40 and correlated with nodal status.
Results
Tumoral expression of D2-40 correspondingly increased with nodal metastasis (p-value=0.261). Further, D2-40 staining was more efficient in detecting individual tumour cells and also characterized the motility factor irrespective of pattern of invasion (p=0.001). Pattern of staining of D2-40 did not show significant association with grades of tumour indicating that motility was an overlooked albeit important component of pattern of invasion in routine histological grading.
Conclusion
D2-40 expression successfully identifies the motility profile of tumour, irrespective of the pattern of invasion. The presence of larger motile islands in the tumour cohort supports the concept of 'Collective Cell Migration'. Podoplanin also aids evasion of immune responses by inducing platelet aggregation over tumour cells thereby favouring distant metastasis. A multivariate model using immunohistochemical staining with D2-40 renders greater sensitivity to the prediction of lymph node metastasis.
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from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2zeRB2A
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