Th17 cells contribute to pulmonary fibrosis and inflammation during chronic kidney disease progression following acute ischemia

Acute kidney injury (AKI) is associated with high mortality rates and predisposes development of chronic kidney disease (CKD). Distant organ damage, particularly in the lung, may contribute to mortality in AKI patients. Animal models of AKI demonstrate an increase in pulmonary infiltration of lymphocytes and reveal an acute compromise of lung function, but the chronic effects of AKI on pulmonary inflammation are unknown. We hypothesized that in response to renal I/R, there is a persistent systemic increase in Th17 cells with potential effects on pulmonary structure and function. Renal ischemia/reperfusion (I/R) injury was performed on rats and CKD progression was hastened by unilateral nephrectomy and exposure to 4.0% sodium diet between 35-63 days post-I/R. Th17 cells in peripheral blood showed a progressive increase up to 63 days following recovery from I/R injury. Infiltration of leukocytes including Th17 cells was also elevated in bronchiolar lavage (BAL) fluid 7 days following I/R and remained elevated for up to 63 days. Lung histology demonstrated an increase in alveolar cellularity and a significant increase in picrosirius red staining. Suppression of lymphocytes with mycophenolate mofetil (MMF) or an IL-17 antagonist significantly reduced Th17 cell infiltration and fibrosis in lung. In addition, tracheal smooth muscle contraction to acetylcholine was significantly enhanced 63-days following I/R relative to sham-operated controls. These data suggest that AKI is associated with a persistent increase in circulating and lung Th17 cells which may promote pulmonary fibrosis and the potential alteration in airway contractility.

from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2AlsNG0