STRO-1 confers myofibroblast transdifferentiation in fibroblasts derived from oral submucous fibrosis

Abstract

Background

STRO-1 is a mesenchymal cell marker present on all clonogenic stromal precursors. Current evidence has indicated that the pathogenesis of fibrotic changes may be mediated by stemness properties. The aim of this study was to investigate the role of STRO-1 in areca quid chewing-associated oral submucous fibrosis (OSF).

Methods

Thirty OSF specimens and ten normal buccal mucosa were examined by immunohistochemistry. The activity of STRO-1 from fibroblasts cultured from normal buccal mucosa (BMFs) and OSF (OSFFs) were measureed and the effects of arecoline, a major areca nut alkaloid, on STRO-1 in BMFs. Compared the activities between sorted STRO-1⁺ cells and STRO-1^- cells from OSFF were measured by collagen gel contraction, migration, invasion abilities, and the expression of α-smooth muscle actin (α-SMA) and pro-α1 (I) chain of type I collagen.

Results

Our results first showed that the expression of STRO-1 was more evident in areca quid chewing-associated OSF than normal buccal mucosa tissues (p<0.05). Arecoline dose-dependently activated the level of STRO-1 in BMFs (p<0.05). The relative expression of STRO-1 was significantly higher in OSFFs compared with BMFs (p<0.05). In addition, the sorted STRO-1⁺ cells from OSFFs exhibited higher collagen gel contraction, migration, and invasion abilities as well as elevated expression of α-SMA and pro-α1 (I) chain of type I collagen than the negative subset (p<0.05).

Conclusion

These findings suggested that the stemness marker STRO-1 may be a crucial factor in the pathogenesis of areca quid chewing-associated OSF.

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from #ORL-AlexandrosSfakianakis via ola Kala on Inoreader http://ift.tt/2jWRIJo