DNA methylation profiles of 22 apoptosis-related genes in odontogenic keratocysts before and after marsupialization

Publication date: Available online 3 August 2017
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Karuza Maria Alves Pereira, Sara Ferreira dos Santos Costa, Núbia Braga Pereira, Marina Gonçalves Diniz, Wagner Henriques Castro, Carolina Cavaliéri Gomes, Ricardo Santiago Gomez
Objective: Odontogenic keratocysts (OKCs) are cystic lesions of the jaws, which tend to recur after treatment. Marsupialization is an effective preliminary treatment for large OKCs. This procedure induces epithelial lining changes in association with Bcl-2 protein expression reduction, but the underlying mechanisms are unknown. The purpose of our study was to compare the methylation profile of apoptosis-related genes of OKCs before and after marsupialization. Study design: We assessed the methylation percentages of the promoter region of 22 apoptosis-related genes in 13 OKCs, marsupialized (m-OKCs) and non-marsupialized lesions by using methylation qPCR array. We validated the expression of genes that showed the greatest differences in methylation percentage between the two groups. Results:LTBR and BCLAF1 showed higher DNA methylation percentage in the m-OKCs, but this difference did not affect gene expression (p > 0.05). The other 20 genes showed similar DNA methylation in both OKC groups. Conclusions: OKC shows a distinct methylation profile after marsupialization, but this is not followed by gene expression alterations.



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