Evaluation of KGF, EGF, VEGF, bcl-2, IL-6 and ki67 expression in oral epithelium adjacent to bisphosphonate-related osteonecrosis and florid osseous dysplasia: a comparative immunohistochemical study

Publication date: Available online 18 September 2017
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Natália Santos Carneiro, Luciana Armada, Débora Lima Pereira, Pablo Agustin Vargas, Márcio Ajudarte Lopes, Fábio Ramôa Pires
Bisphosphonates are associated with impairment of epithelial proliferation and it is suggested that this feature can play a role in the pathogenesis of bisphosphonate-related osteonecrosis of the jaws (ON). Objective: to compare the expression of proliferative markers and apoptosis-associated proteins in the oral mucosa adjacent to bone sequestrum associated with ON and florid osseous dysplasia (OD). Study design: oral mucosal samples derived from surgical procedures performed for treatment of ON and OD (10 cases each) were retrieved. Histological analysis was done on hematoxylin and eosin stained slides; immunohistochemical reactions against epidermal growth factor (EGF), keratinocyte growth factor (KGF), vascular endothelial growth factor (VEGF), Bcl-2 protein, interleukin 6 (IL-6) and Ki-67 antigen were performed with the immunoperoxidase technique. Results: epithelium was hyperplastic in 60% and 22% of ON and OD samples, respectively. Cytoplasmic EGF and KGF expression and Bcl-2, VEGF and IL-6 expressions, and the mean epithelial proliferative index were not statistically different comparing both groups. Membranous EGF expression was more evident in samples showing hyperplastic epithelium. Conclusion: exposure to bisphosphonates did not alter the expression of KGF, EGF, VEGF, Bcl-2, IL-6 and the epithelial proliferating index in comparison with inflamed oral mucosa not exposed to bisphosphonates.



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